The International Microbiome Centre (IMC) at the University of Calgary houses one of the largest germ-free facilities in the world and the only one that has incorporated intra-vital imaging. Constructed in a portion of the basement at the Cumming School of Medicine, the germ-free facility complements the other microbiome platforms—including mass cytometry, genomics, proteomics, metabolomics, bioinformatics, biobanking, and live cell imaging—by providing a model system in which to study the microbiome. The center provides a sterilized housing environment, where researchers explore the microbiome and its impact through adding bacteria to germ-free models and analyzing what those bacteria do to the animals’ immune systems, organs, and overall health.