Whether faced with disrupted research funding streams, the lack of available capital for new construction, or accommodating program expansions, Step One is assessing the condition, capability and utilization of existing research space to chart a path forward. This presentation explores strategies for realigning research space utilization through comprehensive studies assessing the condition, capability, and use of existing facilities. Through case studies from campuses of varying sizes, presenters demonstrate the development of institution-specific metrics to evaluate laboratory spaces, provide insights on more efficient space assignment, and deliver building-specific guidance for aligning research activities with facility capabilities. They introduce an integrated planning framework and decision-making tools, to support both immediate and long-term research priorities, and they advocate for a shift from traditional, department-based space allocation to more flexible, synergy-driven models.